GM-CSF antibody (HRP)

Catalog No. ABIN453899

The Mouse Monoclonal anti-GM-CSF antibody is suitable to detect GM-CSF in samples from Human. It has been validated for EIA.

Quick Overview for GM-CSF antibody (HRP) (ABIN453899)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA)

Clone: 429

Product Details anti-GM-CSF Antibody (HRP)

Specificity: This monoclonal antibody reacts with natural and recombinant human GM-CSF. Does not show any cross reaction with other human Cytokines or Growth Factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGFbeta-1 and TNF-alpha.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: Purified recombinant Human GM-CSF.

Isotype: IgG1

Application Details

Application Notes: ELISA: This HRP-conjugated monoclonal antibody can be used as a Tracer/Detectionantibody in Sandwich ELISA applications for human GM-CSF detection in combination witha Capture antibody (Cat#DM1014). Suggested Capture Coating Dose: approx. 0.3 μg/mL. Substrate: TMB. If the above suggested conditions are followed approximately 2 pg/mL ofGM-CSF in serum/ plasma or medium can be detected with an assay range of 0-500 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.0 ± 0.1 in 50 % Glycerol and 0.01 % Thimerosal as a bacteriostat.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 1437

NCBI Accession: NP_000749

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin