Mouse anti-Human IgG3 Antibody (Biotin)

Catalog No. ABIN457895

Quick Overview for Mouse anti-Human IgG3 Antibody (Biotin) (ABIN457895)

Target: IgG3

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Clone: NI 330 (HP 6050)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Biotin-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific

Characteristics: Biotin/IgG protein molar ratio (B/P) approximately 2.2.

Purification: Purified

Immunogen: Highly purified monoclonal IgG3 isolated from pooled human serum.

Application Details

Application Notes: To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250, in ELISA from 1:100 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG3

Target Type: Antibody