Mouse anti-Human IgG3 Antibody (FITC)

Catalog No. ABIN457896

Quick Overview for Mouse anti-Human IgG3 Antibody (FITC) (ABIN457896)

Target: IgG3

Clonality: Monoclonal

Conjugate: FITC

Application: ELISA, Western Blotting (WB)

Clone: NI 330 (HP 6050)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific

Characteristics: Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.0.

Purification: Purified

Immunogen: Highly purified monoclonal IgG3 isolated from human serum.

Application Details

Application Notes: To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG3

Target Type: Antibody