C5 antibody (FITC)

Catalog No. ABIN458008

This Goat Polyclonal antibody specifically detects C5 in ELISA, IHC (fro), ICC and IFA. It exhibits reactivity toward Human.

Quick Overview for C5 antibody (FITC) (ABIN458008)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This C5 antibody is conjugated to FITC

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)

Product Details anti-C5 Antibody (FITC)

Purpose: Goat anti Human C5, conjugated with FITC

Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to C5 fragment of human complement

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of human serum or plasma. The fluorescent immunoconjugate to human C5 is used to determine the presence and pattern using immunohistochemical staining techniques. Determinations of individual C components can be very useful in defining the exact location of a defect. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: C5 is a component of the complement system. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack-complex. C5 is isolated and purified from pooled normal human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: C5 Fragment of Complement

Background: Human complement C5

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis