Fibrinogen antibody (HRP)

Catalog No. ABIN458673

This anti-Fibrinogen antibody is a Goat Polyclonal antibody detecting Fibrinogen in WB, ELISA, IHC and ICC. Suitable for Rat.

Quick Overview for Fibrinogen antibody (HRP) (ABIN458673)

Target: Fibrinogen

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Fibrinogen Antibody (HRP)

Specificity: The antiserum does not cross-react with any other component of rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to rat fibrinogen

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical techniques for the detection of fibrinogen at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Buffer: Horseradish peroxidase-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details for Fibrinogen

Target: Fibrinogen

Background: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immuno-diffusion (Ouchterlony), using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products