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Fibrinogen antibody

Reactivity: Human, Rat ELISA, IF (cc), IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
Pubmed (2 references) Independent Validation (1 validation)
Catalog No. ABIN673854
$384.62
Plus shipping costs $45.00
100 μL
Shipping to: United States
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  • Target See all Fibrinogen Antibodies
    Fibrinogen
    Reactivity
    • 119
    • 48
    • 47
    • 23
    • 19
    • 16
    • 11
    • 1
    • 1
    Human, Rat
    Host
    • 112
    • 63
    • 40
    • 24
    • 5
    • 2
    Rabbit
    Clonality
    • 202
    • 42
    Polyclonal
    Conjugate
    • 110
    • 35
    • 32
    • 28
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    This Fibrinogen antibody is un-conjugated
    Application
    • 111
    • 91
    • 46
    • 39
    • 38
    • 35
    • 33
    • 27
    • 27
    • 25
    • 25
    • 23
    • 20
    • 19
    • 15
    • 15
    • 9
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Predicted Reactivity
    Mouse
    Purification
    Purified by Protein A.
    Immunogen
    Fibrinogen from human plasma
    Isotype
    IgG
  • Application Notes
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #028531 (ELISA)
    'Independent Validation' Badge
    by
    Alamo Laboratories
    No.
    #028531
    Date
    07/16/2013
    Antigen
    Lot Number
    980680
    Method validated
    ELISA
    Positive Control
    Liver
    Negative Control
    Skeletal muscle
    Notes
    Signal was detected in positive control samples but not in negative control samples.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Fibrinogen antibody
    • Catalog number: ABIN673854
    • Batch number: 980680
    Secondary Antibody
    Full Protocol
    • A 96-well MICROLON® 600 96W High Binding 12 x 8 Clear Strip Microplate was coated with antigen by pipetting 100 µL of sample per well. All samples and standards were assayed in triplicate.
    • The microplate was covered and incubated at 25°C overnight.
    • After overnight coating, plate contents were discarded and wells were washed 3 times with 150 µL of 1 x PBST (PBS, 0.1% Tween-20) per well.
    • Wells were then blocked with 250 µL of 1 x PBS / 0.05% Tween-20 / 1% BSA per well and incubated at 37°C for 2 h.
    • After blocking, plate contents were discarded and wells were washed 3 times with 250 µL of 1 x PBS per well.
    • 100 µL of primary antibody diluted 1:500 in 1 x PBS / 0.05% Tween-20 / 1% BSA was added per well and incubated at 37°C for 2 h.
    • Plate contents were discarded and wells were washed 3 times with 200 µL of 1 x PBST per well.
    • 100 µL of HRP conjugated secondary antibody diluted 1:10000 in 1 x PBS / 0.05% Tween-20 / 1% BSA was added per well and incubated at 37°C for 1 h.
    • Plate contents were discarded and wells were washed 3 times with 200 µL of 1 x PBST per well.
    • 100 µL of TMB (3,3', 5,5"-tetramethylbenzidine) substrate was added per well and incubated at 37°C for 10 min.
    • 100 µL of STOP solution was added per well.
    • The optical density (OD value) of each well was read using a microplate reader set to 450 nm.
    • The triplicate readings for each sample were averaged and the average zero standard optical density subtracted. The corrected average-value was tabulated as Average Absorbance. A standard curve was generated by plotting the mean OD value for each standard on the y-axis against the concentration on the x-axis using Excel. A line of best fit through the points on the graph was used to generate the equation X = (Y-0.0122) / 0.0113.
    • The equation X = (Y-0.0122) / 0.0113 was used to calculate fibrinogen concentrations of the samples based on their Average Absorbance values.
    Experimental Notes
    None
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Chen, Yang, Liao, Wang, Chen, Sun, Huang: "Effect of the duration of UV irradiation on the anticoagulant properties of titanium dioxide films." in: ACS applied materials & interfaces, Vol. 7, Issue 7, pp. 4423-32, (2015) (PubMed).

    Chen, Zhao, Chen, Liao, Yang, Sun, Huang: "The effect of full/partial UV-irradiation of TiO2 films on altering the behavior of fibrinogen and platelets." in: Colloids and surfaces. B, Biointerfaces, Vol. 122, pp. 709-18, (2014) (PubMed).

  • Target
    Fibrinogen
    Abstract
    Fibrinogen Products
    Synonyms
    fib2 antibody, MGC53620 antibody, fibrinogen antibody, sb:cb892 antibody, wu:fa55c12 antibody, wu:fb20e08 antibody, wu:fb59b03 antibody, wu:fi39d04 antibody, zgc:92117 antibody, fibrinogen alpha chain antibody, fibrinogen gamma chain antibody, fga antibody, FGA antibody, LOC698244 antibody, CpipJ_CPIJ010090 antibody, FGG antibody
    Background

    Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

    Subcellular location: Secreted

    Synonyms: Fib2, Fibrinogen alpha chain, FGA

    Gene ID
    2243
    UniProt
    P02671
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