Fibrinogen antibody (Biotin)

Catalog No. ABIN458358

Cited in 2+ publications. The Biotin-conjugated Goat Polyclonal anti-Fibrinogen antibody (ABIN458358) specifically detects Fibrinogen in WB, ELISA, IHC (p), ICC and DB. The antibody is reactive with Mouse samples.

Quick Overview for Fibrinogen antibody (Biotin) (ABIN458358)

Target: Fibrinogen

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-Fibrinogen Antibody (Biotin)

Purpose: Goat anti Mouse fibrinogen, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse fibrinogen

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in mouse cells, tissues and body fluids in immunocytochemical and immunohistochemical assays, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

References for anti-Fibrinogen antibody (Biotin) (ABIN458358)

Díaz-Trelles, Scimia, Bushway, Tran, Monosov, Monosov, Peterson, Rentschler, Cabrales, Ruiz-Lozano, Mercola: "Notch-independent RBPJ controls angiogenesis in the adult heart." in: Nature communications, Vol. 7, pp. 12088, (2016) (PubMed).

Lin, Kwong, Warren, Wood, Bhatia: "Nanoparticles that sense thrombin activity as synthetic urinary biomarkers of thrombosis." in: ACS nano, Vol. 7, Issue 10, pp. 9001-9, (2014) (PubMed).

Target Details for Fibrinogen

Target: Fibrinogen