Fibrinogen antibody

Catalog No. ABIN458743

This Goat Polyclonal antibody specifically detects Fibrinogen in WB, ELISA, DB and IFA. It exhibits reactivity toward Rat. It has been mentioned in 2+ publications

Quick Overview for Fibrinogen antibody (ABIN458743)

Target: Fibrinogen

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Product Details anti-Fibrinogen Antibody

Purpose: Goat anti Rat fibrinogen

Specificity: Purified IgG fraction of polyclonal Goat antiSerum to Rat fibrinogen.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is restricted to rat fibrinogen molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in rat cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Purification: Purified IgG fraction from antiserum

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

References for anti-Fibrinogen antibody (ABIN458743)

Hartmannsberger, Ben-Kraiem, Kramer, Guidolin, Kazerani, Doppler, Thomas, Gurke, Sisignano, Kalelkar, García, Monje, Sammeth, Nusrat, Brack, Krug, Sommer, Rittner: "TAM receptors mediate the Fpr2-driven pain resolution and fibrinolysis after nerve injury." in: Acta neuropathologica, Vol. 149, Issue 1, pp. 1, (2024) (PubMed).

Chen, Schmidt, Schürger, Hankir, Krug, Rittner: "Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury." in: International journal of molecular sciences, Vol. 22, Issue 18, (2021) (PubMed).

Target Details for Fibrinogen

Target: Fibrinogen

UniProt: P06399, P14480, P02680