Erythrocytes antibody

Catalog No. ABIN458786

Product Details

Target: Erythrocytes

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Screening Assay (ScA)

Specificity: Homologous proteins of different species frequently share antigenic determinants. has not been tested.

Characteristics: IgG fraction of polyclonal rabbit antiserum to sheep erythrocytes

Purification: Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x10 8 RBC/ml, not less than 1:3,000

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details

Target: Erythrocytes