beta Amyloid antibody

Catalog No. ABIN459388

This Mouse Monoclonal antibody specifically detects beta Amyloid in ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for beta Amyloid antibody (ABIN459388)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Amyloid antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: OMAB

Product Details anti-beta Amyloid Antibody

Purpose: Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB)

Cross-Reactivity (Details): Human Abeta oligomers only
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Rat

Purification: Affinity purified

Immunogen: Partly aggregated

Isotype: IgM

Application Details

Application Notes: Coating antibody at 2 μg/mL (ELISA), 1: 1000 (IF), 1 : 500 (IHC)

Comment:

OMAB antibody is a versatile tool within research of Alzheimer's disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Affinity purified in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Amyloid beta oligomer-specific

Background:

Background: Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Additional information: OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

Molecular Weight: 4.5 kDa

Pathways: Inflammasome