Caspase 1 antibody

Catalog No. ABIN461892

The Rabbit Polyclonal anti-Caspase 1 antibody (ABIN461892) specifically detects Caspase 1 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Caspase 1 antibody (ABIN461892)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 1 Antibody

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1 / Caspase 1

Background: Name/Gene ID: CASP1
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome