SPHK1 antibody (C-Term)

Catalog No. ABIN4620336

The Rabbit Polyclonal anti-SPHK1 antibody has been validated for WB and EIA. It is suitable to detect SPHK1 in samples from Human, Mouse, Rat and Cow. There are 8+ publications available.

Quick Overview for SPHK1 antibody (C-Term) (ABIN4620336)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SPHK1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to SPHK1.

Purification: Affinity Purified

Immunogen: Synthetic peptide derived from the C-terminal of Sphingosine Kinase 1

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 400. ELISA. POSITIVE CONTROL: Madin-Darby Bovine Kidney (MDBK) cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped on dry ice. Store (in aliquots) at -20 °C only.

References for anti-SPHK1 antibody (ABIN4620336)

Moles, Urbán, Jiménez-Díaz, Viera-Morilla, Angulo-Barturen, Busquets, Fernàndez-Busquets: "Immunoliposome-mediated drug delivery to Plasmodium-infected and non-infected red blood cells as a dual therapeutic/prophylactic antimalarial strategy." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 210, pp. 217-29, (2016) (PubMed).

Van Vaerenbergh, De Smet, Rafei-Shamsabadi, Blank, Spillner, Ebo, Devreese, Jakob, de Graaf: "IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology." in: Molecular immunology, Vol. 63, Issue 2, pp. 449-55, (2015) (PubMed).

You, Sandalcioglu, Dammann, Felbor, Sure, Zhu: "Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations." in: Journal of cellular and molecular medicine, Vol. 17, Issue 3, pp. 407-18, (2013) (PubMed).

DAngelo, Polishchuk, Di Tullio, Santoro, Di Campli, Godi, West, Bielawski, Chuang, van der Spoel, Platt, Hannun, Polishchuk, Mattjus, De Matteis: "Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide." in: Nature, Vol. 449, Issue 7158, pp. 62-7, (2007) (PubMed).

Kelly, Allport, Yu, Sinh, Sage, Gerszten, Weissleder: "SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration." in: Journal of leukocyte biology, Vol. 81, Issue 3, pp. 748-56, (2007) (PubMed).

Yang, Castle, Hanidu, Stevens, Yu, Li, Stearns, Papov, Rajotte, Li: "Sphingosine kinase 1 is a negative regulator of CD4+ Th1 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 10, pp. 6580-8, (2005) (PubMed).

Zhao, Usatyuk, Cummings, Saatian, He, Watkins, Morris, Spannhake, Brindley, Natarajan: "Lipid phosphate phosphatase-1 regulates lysophosphatidic acid-induced calcium release, NF-kappaB activation and interleukin-8 secretion in human bronchial epithelial cells." in: The Biochemical journal, Vol. 385, Issue Pt 2, pp. 493-502, (2005) (PubMed).

Katsuma, Hada, Ueda, Shiojima, Hirasawa, Tanoue, Takagaki, Ohgi, Yano, Tsujimoto: "Signalling mechanisms in sphingosine 1-phosphate-promoted mesangial cell proliferation." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1217-30, (2003) (PubMed).

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Background: Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g.S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).Synonyms: SPHK, SPK, SPK 1, SPK-1

Gene ID: 8877

NCBI Accession: NP_001136073

UniProt: Q9NYA1

Pathways: VEGF Signaling