TUBB antibody

Catalog No. ABIN462130

This Mouse Monoclonal antibody specifically detects TUBB in WB, IHC, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Chicken, Cow and Frog.

Quick Overview for TUBB antibody (ABIN462130)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Cow, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TUBB Antibody

Specificity: Recognizes human Tubulin, beta. Species cross-reactivity: Bovine, rabbit, mouse, chicken, rat, frog, moth and wheat.

Purification: Protein G purified

Immunogen: Purified tubulin, 55kD. Cellular Localization: Cytoplasmic.

Isotype: IgG3

Application Details

Application Notes: Approved: IF, IHC, IHC-P (10 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 0.5-1 μg/mL for 2 hours at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB / Beta Tubulin

Background: Name/Gene ID: TUBB

Synonyms: TUBB, Beta Tubulin, Beta 5-tubulin, Beta-4 tubulin, Beta1-tubulin, M40, Tubulin beta-5 chain, Beta Ib tubulin, Class I beta-tubulin, OK/SW-cl.56, TUBB5, Tubulin beta chain, Tubulin beta polypeptide, Tubulin beta-1 chain, Tubulin, beta class I, Tubulin, beta polypeptide

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase