Tyrosine Hydroxylase antibody

Catalog No. ABIN462131

The Rabbit Polyclonal anti-Tyrosine Hydroxylase antibody has been validated for WB, IHC, IF, ELISA, IP, IHC (p) and IHC (fro). It is suitable to detect Tyrosine Hydroxylase in samples from Human, Rat, Mouse, Cat and Ferret.

Quick Overview for Tyrosine Hydroxylase antibody (ABIN462131)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Human, Rat, Mouse, Cat, Ferret

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tyrosine Hydroxylase Antibody

Brand: IHC-plus™

Specificity: Recognizes tyrosine hydroxylase. Species cross-reactivity: Mouse, human, ferret, feline and Aplysia (marine mollusc). Because Tyrosine Hydroxylase is highly conserved, this antibody is expected to cross-react with most mammalian species and many non-mammalian species.

Purification: Immunoaffinity purified

Immunogen: Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (1:100), IP, WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:1000 on 10 μg of PC12 lysates. Immunofluorescence: 1:100-1:1000. Fixed with 4 % PFA overnight at 4C.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: TH / Tyrosine Hydroxylase

Background: Name/Gene ID: TH
Family: Monooxygenase

Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

Gene ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour