Cardiac Troponin T2 antibody (Exon 10)

Catalog No. ABIN462337

This Mouse Monoclonal antibody specifically detects Cardiac Troponin T2 in IHC, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Chicken, Horse, Sheep and Monkey.

Quick Overview for Cardiac Troponin T2 antibody (Exon 10) (ABIN462337)

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Chicken, Horse, Sheep, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cardiac Troponin T2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cardiac Troponin T2 Antibody

Binding Specificity: Exon 10

Specificity: Recognizes the cardiac isoform of rabbit Troponin T (cTnT). Species cross-reactivity: Canine, chicken, goldfish, guinea pig, human, mouse, porcine, rat, sparrow. Does not react with frog.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Chicken, Platypus, Lizard (100%) Hamster, Opossum (90%).

Purification: Protein G purified

Immunogen: Purified rabbit cardiac troponin T isoform (TnT4R). Epitope: FMPNLVPPKI (5' region of the cardiac gene exon 10). Cellular Localization: Mostly over the I bands in myofibrils. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Chicken, Platypus, Lizard (100%), Hamster, Opossum (90%).

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. The antibody is also suitable for electron microscopy and immunofluorescence.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cardiac Troponin T2

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Alternative Name: TNNT2 / CTNT

Background: Name/Gene ID: TNNT2

Synonyms: TNNT2, Cardiomyopathy, hypertrophic 2, CMPD2, CMD1D, CMH2, Cardiac muscle troponin T, Cardiac Troponin T, LVNC6, TnTC, Troponin T type 2 (cardiac), Troponin T, cardiac muscle, Troponin T2, cardiac, CTnT, RCM3

Gene ID: 7139

UniProt: P45379