SOD2 antibody

Catalog No. ABIN462380

The Rabbit Polyclonal anti-SOD2 antibody has been validated for WB, IHC, IP, IHC (p) and FACS. It is suitable to detect SOD2 in samples from Human, Rat, Mouse, Pig, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Hamster, Xenopus laevis and Drosophila melanogaster.

Quick Overview for SOD2 antibody (ABIN462380)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Rat, Mouse, Pig, Sheep, Dog, Rabbit, Cow, Guinea Pig, Monkey, Hamster, Xenopus laevis, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-SOD2 Antibody

Brand: IHC-plus™

Purification: Immunoaffinity purified

Immunogen: Native rat Mn SOD protein.

Application Details

Application Notes: Approved: Flo (1:1000), IHC, IHC-P (10 μg/mL), IP (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sodium phosphate buffer,  pH 7.0, 15 mg/mL BSA, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: MNSOD / SOD2

Background: Name/Gene ID: SOD2

Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase

Gene ID: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling