CHRNA7 antibody

Catalog No. ABIN462402

The Goat Polyclonal anti-CHRNA7 antibody (ABIN462402) specifically detects CHRNA7 in IHC, ELISA and IHC (p). The antibody is reactive with Human, Cow, Chicken, Monkey, Dog, Pig and Horse samples.

Quick Overview for CHRNA7 antibody (ABIN462402)

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivity: Human, Cow, Chicken, Monkey, Dog, Pig, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNA7 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRNA7 Antibody

Specificity: Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%) Mouse, Rat, Hamster, Rabbit (94%) Marmoset, Xenopus, Zebrafish (88%) Bat, Platypus (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KRPGEDKVRPACQHKQ from an internal region of human CHRNA7 (NP_000737.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%), Mouse, Rat, Hamster, Rabbit (94%), Marmoset, Xenopus, Zebrafish (88%), Bat, Platypus (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHRNA7

Target: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Alternative Name: CHRNA7

Background: Name/Gene ID: CHRNA7
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA7, AChR-alpha7, Acra 7, CHRNA7-2, NAChR alpha 7, NACHRA7

Gene ID: 1139

NCBI Accession: NP_000737

UniProt: P36544

Pathways: Synaptic Membrane