TRAIL antibody
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- Target See all TRAIL (TNFSF10) Antibodies
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TRAIL antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human TRAIL/Apo2L
- Purification
- Protein A purified
- Immunogen
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E.coli derived recombinant Human TRAIL/Apo2L.
Type of Immunogen: Cell extract - Isotype
- IgG1
- Top Product
- Discover our top product TNFSF10 Primary Antibody
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- Application Notes
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Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TRAIL/Apo2L (10 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human sTRAIL/APO2L when used with biotinylated antigen affinity purified anti-human sTRAIL/APO2L (LS-C104497) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1 ng/lane, under reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in Sterile water to a concentration of 1 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Alternative Name
- TNFSF10 / TRAIL (TNFSF10 Products)
- Synonyms
- APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody
- Background
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Name/Gene ID: TNFSF10
Family: TNF
Synonyms: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253 antigen, TRAIL, Protein TRAIL, TL2 - Gene ID
- 8743
- UniProt
- P50591
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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