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CD22 antibody

This anti-CD22 antibody is a Rabbit Polyclonal antibody detecting CD22 in WB and ELISA. Suitable for Human.
Catalog No. ABIN465261

Quick Overview for CD22 antibody (ABIN465261)

Target

See all CD22 Antibodies
CD22 (CD22 Molecule (CD22))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This CD22 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA
  • Specificity

    Human sCD22

    Purification

    Immunoaffinity purified

    Immunogen

    CHO cell derived recombinant Human sCD22.

    Type of Immunogen: Cell extract
  • Application Notes

    Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: ELISA: To detect hCD22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (LS-C104523) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Western Blot: To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Sterile water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS,  pH 7.2

    Handling Advice

    Avoid freeze/thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target

    CD22 (CD22 Molecule (CD22))

    Alternative Name

    CD22

    Background

    Name/Gene ID: CD22
    Family: Immunoglobulin

    Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

    Gene ID

    933

    UniProt

    P20273
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