CD22 antibody (Biotin)

Catalog No. ABIN465292

The Biotin-conjugated Rabbit Polyclonal anti-CD22 antibody (ABIN465292) specifically detects CD22 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD22 antibody (Biotin) (ABIN465292)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD22 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CD22 Antibody (Biotin)

Specificity: Human sCD22

Purification: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human sCD22.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hCD22 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. To detect hCD22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human CD22 (LS-C104507) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Western Blot: To detect hCD22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCD22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

Gene ID: 933

UniProt: P20273