TGFB1 antibody

Catalog No. ABIN465317

The Mouse Monoclonal anti-TGFB1 antibody has been validated for WB and ELISA. It is suitable to detect TGFB1 in samples from Human, Mouse and Monkey.

Quick Overview for TGFB1 antibody (ABIN465317)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TGFB1 Antibody

Specificity: Human TGF-beta1

Purification: Protein G purified

Immunogen: Hi-5 Insect cell derived recombinant Human TGF-beta1.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 100 pg/mL of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents. Western Blot: To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2-4 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2-4 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 / TGF Beta 1

Background: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gene ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints