VEGFA antibody

Catalog No. ABIN465335

The Rabbit Polyclonal anti-VEGFA antibody is suitable to detect VEGFA in samples from Rat. It has been validated for WB, ELISA and Neut.

Quick Overview for VEGFA antibody (ABIN465335)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-VEGFA Antibody

Specificity: Rat VEGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Rat VEGF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat VEGF (LS-C104553) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA / VEGF

Background: Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling