IL-13 antibody (Biotin)

Catalog No. ABIN465436

This anti-IL-13 antibody is a Rabbit Polyclonal antibody detecting IL-13 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for IL-13 antibody (Biotin) (ABIN465436)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-13 Antibody (Biotin)

Specificity: Mouse IL-13

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-13.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-13 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-13. To detect mIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-13 (LS-C104574) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-13. Western Blot: To detect mIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background: Name/Gene ID: IL13
Family: Hormone

Synonyms: IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13

Gene ID: 3596

UniProt: P35225

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport