IL-33 antibody

Catalog No. ABIN465633

The Rabbit Polyclonal anti-IL-33 antibody is suitable to detect IL-33 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for IL-33 antibody (ABIN465633)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-33 Antibody

Specificity: Human IL-33

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-33.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL33

Background: Name/Gene ID: IL33
Family: Interleukin

Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response