IL-5 antibody
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- Target See all IL-5 (IL5) Antibodies
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL-5 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Human IL-5
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human IL-5.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL5 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-5 (5 ng/mL), a concentration of 0.05-0.15 μg/mL of this antibody is required. ELISA: To detect Human IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (LS-C104567) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL-5 (IL5) (Interleukin 5 (IL5))
- Alternative Name
- IL5 (IL5 Products)
- Synonyms
- EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody
- Background
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Name/Gene ID: IL5
Family: Hormone
Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF - Gene ID
- 3567
- UniProt
- P05113
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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