IL-5 antibody

Catalog No. ABIN465636

The Rabbit Polyclonal anti-IL-5 antibody (ABIN465636) specifically detects IL-5 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL-5 antibody (ABIN465636)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-5 Antibody

Specificity: Human IL-5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-5 (5 ng/mL), a concentration of 0.05-0.15 μg/mL of this antibody is required. ELISA: To detect Human IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (LS-C104567) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Background: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

Gene ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour