Thrombopoietin antibody

Catalog No. ABIN465841

Product Details anti-Thrombopoietin Antibody

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human TPO

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TPO.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (4.2 - 6.3 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTPO (50 ng/mL), a concentration of 05-08 μg/mL of this antibody is required. ELISA: To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (LS-C104660) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: THPO / TPO / Thrombopoietin (THPO Products)

Synonyms: MGDF antibody, MKCSF antibody, ML antibody, MPLLG antibody, THCYT1 antibody, TPO antibody, Mgdf antibody, Ml antibody, Mpllg antibody, Tpo antibody, Tpo1 antibody, Tpo2 antibody, Tpo3 antibody, Tpo4 antibody, tpo antibody, LOC100231762 antibody, thrombopoietin antibody, THPO antibody, Thpo antibody, thpo antibody

Background: Name/Gene ID: THPO
Family: Hormone

Synonyms: THPO, C-mpl ligand, MGDF, MPL ligand, THCYT1, Thrombopoietin nirs variant 1, MKCSF, MPLLG, Thrombopoietin

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity