IL-6 antibody (Biotin)

Catalog No. ABIN465886

This anti-IL-6 antibody is a Rabbit Polyclonal antibody detecting IL-6 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for IL-6 antibody (Biotin) (ABIN465886)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-6 Antibody (Biotin)

Specificity: Mouse IL-6

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-6.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine IL-6 by direct ELISA (using 100 ml/well) a concentration of approximately 1 μg/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. To detect Murine IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-6 (LS-C104805) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: Name/Gene ID: IL6
Family: Hormone

Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome