RHOA antibody (AA 1-155)

Catalog No. ABIN470631

The Mouse Monoclonal anti-RHOA antibody (ABIN470631) specifically detects RHOA in WB, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RHOA antibody (AA 1-155) (ABIN470631)

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RHOA Antibody

Binding Specificity: AA 1-155

Specificity: Recognizes human Rho (-A, -B, -C), Mr 22kD. Species cross-reactivity: rat and mouse.

Purification: Protein G purified

Immunogen: Recombinant human RhoA containing amino acids 1-155.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IF, WB

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Reported to detect Rho in cells via immunofluorescence.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RHOA

Background: Name/Gene ID: RHOA
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOA, ARH12, ARHA, Aplysia ras-related homolog 12, H12, Oncogene RHO H12, Rho cDNA clone 12, RHO12, RHOH12, Ras homolog family member A, Small GTP binding protein RhoA, Transforming protein RhoA

Gene ID: 387

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome