FZD2 antibody (Extracellular Domain)

Catalog No. ABIN478745

The Rat Monoclonal anti-FZD2 antibody has been validated for WB and ELISA. It is suitable to detect FZD2 in samples from Mouse.

Quick Overview for FZD2 antibody (Extracellular Domain) (ABIN478745)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FZD2 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse Frizzled-2. No cross-reactivity was observed with rmFrizzled-1, -3, -4, -6, -7, -8 or rhFrizzled-5.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-2 extracellular domain.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-2. Using a colorimetric detection system, the detection limit for rmFrizzled-2 is ~25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Use at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled-2 is ~5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FSD2 / Frizzled 2

Background: Name/Gene ID: FZD2
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2

Gene ID: 2535

UniProt: Q14332

Pathways: WNT Signaling