SR Proteins antibody

Catalog No. ABIN480267

The Mouse Monoclonal anti-SR Proteins antibody (ABIN480267) specifically detects SR Proteins in IF, ELISA and WB. The antibody is reactive with Human and Xenopus laevis samples.

Quick Overview for SR Proteins antibody (ABIN480267)

Target: SR Proteins

Reactivity: Human, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Product Details

Specificity: Detects the non-snRNP splicing factors termed SR proteins. Recognizes all members of the SR protein family. However, the epitope is phosphorylation-dependent so care must be taken to avoid dephosphorylation. Species cross-reactivity: Human and Xenopus.

Purification: Protein A purified

Immunogen: Mass-isolated nuclei from Xenopus Laevis oocytes.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF, WB

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot. Binding activity is confirmed by Western blotting of HeLa cell extracts and HeLa cell nuclear extracts. ELISA: 0.1-1.0 μg/mL. Immunofluorescence1,2:2-3 μg/mL. Western Blot1:0.4-10 μg/mL.

Comment:

Target Species of Antibody: Xenopus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: SR Proteins