TAG-72 antibody (HRP)

Catalog No. ABIN480371

The Mouse Monoclonal anti-TAG-72 antibody is suitable to detect TAG-72 in samples from Human and Rat. It has been validated for IHC (p), IHC, FACS, WB and IP.

Quick Overview for TAG-72 antibody (HRP) (ABIN480371)

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAG-72 antibody is conjugated to HRP

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-TAG-72 Antibody (HRP)

Specificity: Recognizes human TAG-72. Species cross-reactivity: bovine, canine and rat.

Purification: Protein G purified

Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis and purified TAG-72 protein. MW: >1000kD.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (0.2 - 0.4 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation, Radioimaging and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunoprecipitation: Use Protein G, 2 μg/mg protein lysate. Immunohistochemistry, Formalin-fixed, paraffin-embedded: 0.2-0.4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Ls174T cells. Colon Carcinoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No stabilizing proteins added. No preservative added.

Preservative: Without preservative

Target Details for TAG-72

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72

Background: Name/Gene ID: TAG-72

Synonyms: TAG-72