PPP1R12A antibody (pThr850)

Catalog No. ABIN481150

This Rabbit Polyclonal antibody specifically detects PPP1R12A in WB. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Zebrafish (Danio rerio), Monkey, Rabbit, Dog and Horse.

Quick Overview for PPP1R12A antibody (pThr850) (ABIN481150)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human, Mouse, Rat, Chicken, Zebrafish (Danio rerio), Monkey, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP1R12A Antibody

Binding Specificity: AA 845-855, pThr850

Specificity: Recognizes recombinant human truncated MYPT1 phosphorylated on Thr850, MW ~80kD. Species cross-reactivity: mouse, rat and chicken.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa845-855 (C-EKRRS[pT]GVSFW) of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL. Detects MYPT1 in C2C12 lysates treated with pervanadate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: PPP1R12A / MYPT1

Background: Name/Gene ID: PPP1R12A

Synonyms: PPP1R12A, M130, MYPT1, MBS, Myosin binding subunit

Gene ID: 4659

UniProt: O14974

Pathways: M Phase