Endothelial Cells antibody (Biotin)

Catalog No. ABIN481662

The Biotin-conjugated Rat Monoclonal anti-Endothelial Cells antibody (Clone 1F10) (ABIN481662) specifically detects Endothelial Cells in IHC (fro) and IHC. The antibody is reactive with Human samples.

Quick Overview for Endothelial Cells antibody (Biotin) (ABIN481662)

Target: Endothelial Cells

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Endothelial Cells antibody is conjugated to Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Clone: 1F10

Product Details anti-Endothelial Cells Antibody (Biotin)

Specificity: The antigen and epitope have not been further characterized. Antigen distribution: Isolated cells: The antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence on the antigen expression. Specificity: Human: continuous endothelial cells. Other: negative in mouse negative, other species not tested.

Purification: Affinity purified

Immunogen: cultured HUVECs

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:400)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for Endothelial Cells

Target: Endothelial Cells