EPOR antibody (pTyr368)

Catalog No. ABIN482201

The Rabbit Polyclonal anti-EPOR antibody is suitable to detect EPOR in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and IF.

Quick Overview for EPOR antibody (pTyr368) (ABIN482201)

Target: EPOR (Erythropoietin Receptor (EPOR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-EPOR Antibody

Binding Specificity: AA 341-390, pTyr368

Purpose: EpoR (phospho Tyr368) Antibody

Specificity: Phospho-EpoR (Y368) Polyclonal Antibody detects endogenous levels of EpoR protein only when phosphorylated at Y368.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Epo-R around the phosphorylation site of Tyr368. AA range:341-390

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for EPOR

Target: EPOR (Erythropoietin Receptor (EPOR))

Alternative Name: Epo-R

Background:

Synonyms: EPOR, Erythropoietin receptor, EPO-R

Background: This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

Gene Name: EPOR

Protein Name: Erythropoietin receptor

Molecular Weight: 55 kDa

Gene ID: 2057

OMIM: 133100

UniProt: P19235

Pathways: JAK-STAT Signaling