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Vimentin antibody (pSer83)

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Western blot analysis of extracts from 293 cells treated with paclitaxel 1uM 24h, using Vimentin (Phospho-Ser83) Antibody. (Vimentin antibody (pSer83))

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser83) Antibody. (Vimentin antibody (pSer83))

IHC

2 images

Catalog No. ABIN482371

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Quick Overview for Vimentin antibody (pSer83) (ABIN482371)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human, Rat, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Discover our top product VIM Primary Antibody

Binding Specificity
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AA 56-105, pSer83

Specificity

Vimentin (Phospho-Ser83) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser83.
PhosphorylationH:S83 M:S83 R:S83

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser83.

Isotype

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 210-466) antibody
VIM Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5D7 unconjugated

Application Notes

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Synonyms: VIME, vimentin
NCBI Gene Symbol: VIM

Molecular Weight

53 kDa

Gene ID

7431

OMIM

193060

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis