LIME antibody (AA 86-202)

Catalog No. ABIN487426

This Mouse Monoclonal antibody specifically detects LIME in WB and IP. It exhibits reactivity toward Mouse.

Quick Overview for LIME antibody (AA 86-202) (ABIN487426)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: MLIME-05

Product Details anti-LIME Antibody

Binding Specificity: AA 86-202

Specificity: This antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Characteristics: Synonyms: LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule,Lck-interacting transmembrane adapter 1

Purification: Protein A affinity chromatography

Immunogen: Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation (use murine splenic T cells as positive control). Western blot: 1-5 μg/mL (reducing conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg /ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1

Background: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

Gene ID: 72699

UniProt: Q9EQR5