ITGA7 antibody

Catalog No. ABIN487462

Product Details anti-ITGA7 Antibody

Target: ITGA7 (Integrin, alpha 7 (ITGA7))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein-A Agarose Chromatography.

Immunogen: Mouse myoblasts. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2/0 with Integrinalpha-7 knockout C57/B6 Mouse splenocyte

Clone: 3C12

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Immunocytochemistry. Positive Control: C2C12. Detailed Procedure is provided in the following Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Analysis for Adherent CellsWe usually use Fisher tubes or equivalents as reaction tubes for all steps after 2). 1) Detach the cells from the culture dish by using cell dissociation buffer2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN3]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 6) Add 40 µL of the primary antibody at the concentration suggested in the APPLICATIONS,diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 8) Add 30 µL of 1: 100 FITC conjugated anti-mouse IgG diluted with the washing buffer. Mixwell and incubate for 15 minutes at room temperature. 9) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 10) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control for Flow Cytometry: C2C12Immunocytochemistry1) Add the primary antibody diluted with PBS as suggest in the APPLICATIONS onto thecells and incubate for 1 hour at RT. (Optimization of antibody concentration or incubationcondition are recommended if necessary. )2) Prepare a wash container such as a 500 mL beaker with a magnetic stirrer. Then washthe cultured cells on the glass slide by soaking the slide with plenty of PBS in the washcontainer for 5 minutes. Take care not to touch the cells. Repeat wash once more. 3) Add 30 µL of 1: 40 FITC conjugated anti-mouse IgG diluted with PBS onto the cells. Incubate for 30 minutes at RT. Keep out light by covering with aluminum foil. 4) Wash the slide in plenty of PBS as in step 2). 5) Wipe excess liquid from slide but take care not to touch the cells. Never leave the cellsto dry. 6) Promptly add PermafluorTM aqueous mounting medium onto the slide, then put a coverslip on it.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ITGA7

Target: ITGA7 (Integrin, alpha 7 (ITGA7))

Alternative Name: Integrin alpha-7 / ITGA7 (ITGA7 Products)

Synonyms: [a]7 antibody, alpha7 antibody, integrin alpha 7 antibody, integrin subunit alpha 7 antibody, Itga7 antibody, ITGA7 antibody

Background: The integrin family of adhesion molecules participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of an alpha subunit and a beta subunit. Both alpha and beta subunits are transmembrane proteins with large extracellular domains (>100 kDa for alpha subunit and >75 kDa for beta subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the beta-4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed

UniProt: Q61738

Pathways: Integrin Complex