Toc75 antibody (Chloroplastic, Domain 3)

Catalog No. ABIN5684021

Product Details anti-Toc75 Antibody

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Binding Specificity: Chloroplastic, Domain 3

Reactivity: Pisum sativum, Synechococcus sp., Thermosynechococcus, Nicotiana tabacum, Spinach, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Toc75 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Purification: serum

Immunogen: psTOC75, Predicted POTRA Domain #3, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

Application Details

Application Notes: Recommended Dilution: 1 : 2000 - 1 : 25 000, 1 : 100 000 (WB), 1 : 500 (FACS). 1 : 100 (LSCM).

Comment:

antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Target Details for Toc75

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Abstract: Toc75 Products

Background: AGI Code: At3g46740
OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Molecular Weight: expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

UniProt: Q43715