PPI antibody

Catalog No. ABIN488549

The Rabbit Polyclonal anti-PPI antibody is suitable to detect PPI in samples from Papaya. It has been validated for IHC, ELISA, WB and IF.

Quick Overview for PPI antibody (ABIN488549)

Target: PPI

Reactivity: Papaya

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details

Purpose: Anti-PPI | papain

Cross-Reactivity (Details): Carica papaya
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Carica papaya

Purification: Purified IgG

Immunogen: Native papain isolated and purified from Carica papaya

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Comment:

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1 mL of sterile destilled water

Buffer: Purified IgG in PBS.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: PPI

Background:

Background: Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.

Additional information: Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition

Molecular Weight: 38.9 kDa