PPI antibody (HRP)

Catalog No. ABIN7565802

The HRP-conjugated Goat Polyclonal anti-PPI antibody (ABIN7565802) specifically detects PPI in IHC, ELISA, WB and DB. The antibody is reactive with Papaya samples.

Quick Overview for PPI antibody (HRP) (ABIN7565802)

Target: PPI

Reactivity: Papaya

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Dot Blot (DB)

Product Details

Purpose: Papain Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

Characteristics: Synonyms: goat anti-Papain Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

Purification: Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:200- 1:1,000 Western Blot Dilution: 1:500- 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: PPI

Background: Background: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

UniProt: P00784