ABHD5 antibody (AA 169-349)

Catalog No. ABIN4886411

Product Details anti-ABHD5 Antibody

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Binding Specificity: AA 169-349

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABHD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(ABHD5) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for 1-acylglycerol-3-phosphate O-acyltransferase ABHD5(ABHD5) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: abhydrolase domain containing 5
Protein Name: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). Human Abhd5 shares 96.7% amino acid (aa) sequence identity with both mouse and rat Abhd5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ABHD5

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Alternative Name: ABHD5 (ABHD5 Products)

Synonyms: ABHD5 antibody, cds antibody, cgi58 antibody, iecn2 antibody, ncie2 antibody, abhd5 antibody, CDS antibody, CGI58 antibody, IECN2 antibody, NCIE2 antibody, 1300003D03Rik antibody, 2010002J10Rik antibody, CGI-58 antibody, IECN5 antibody, abhydrolase domain containing 5 antibody, abhydrolase domain containing 5a antibody, abhydrolase domain containing 5 L homeolog antibody, ABHD5 antibody, abhd5 antibody, abhd5a antibody, Abhd5 antibody, abhd5.L antibody

Background: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 is an enzyme that in humans is encoded by the ABHD5 gene. The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated withChanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Synonyms: ABHD5 | CDS | CGI 58 | CGI58 | CGI58 protein | IECN2 | NCIE2 | Q8WTS1

Gene ID: 51099

Pathways: Lipid Metabolism