ACTN3 antibody (C-Term)

Catalog No. ABIN4886420

The Rabbit Polyclonal anti-ACTN3 antibody is suitable to detect ACTN3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for ACTN3 antibody (C-Term) (ABIN4886420)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ACTN3 Antibody

Binding Specificity: AA 574-617, C-Term

Purpose: Anti-ACTN3 Antibody

Sequence: EADRERGAIM GIQGEIQKIC QTYGLRPCST NPYITLSPQD INT

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ACTN3 Antibody (ABIN4886420). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: ACTN3

Background:

Synonyms: Alpha-actinin-3,Alpha-actinin skeletal muscle isoform 3,F-actin cross-linking protein,ACTN3,

Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers. .

Background: Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants, the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Molecular Weight: 103 kDa

Gene ID: 89

UniProt: Q08043

Pathways: Cell-Cell Junction Organization