ADO antibody (AA 49-261)

Catalog No. ABIN4886431

Product Details anti-ADO Antibody

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Binding Specificity: AA 49-261

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADO antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for 2-aminoethanethiol dioxygenase(ADO) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for 2-aminoethanethiol dioxygenase(ADO) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: 2-aminoethanethiol dioxygenase
Protein Name: 2-aminoethanethiol dioxygenase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Alternative Name: ADO (ADO Products)

Synonyms: C10orf22 antibody, Gm237 antibody, RGD1308233 antibody, wu:fc32g01 antibody, wu:fc52c12 antibody, zgc:101580 antibody, 2-aminoethanethiol dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase a antibody, ADO antibody, Ado antibody, LOC100351598 antibody, adoa antibody

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Synonyms: ADO | C10orf22 | Cysteamine dioxygenase | Q96SZ5

Gene ID: 84890