APOB antibody (AA 246-450)

Catalog No. ABIN4886467

Product Details anti-APOB Antibody

Target: APOB (Apolipoprotein B (APOB))

Binding Specificity: AA 246-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Apolipoprotein B-100(APOB) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Apolipoprotein B-100(APOB) detection. Tested with WB, IHC-P in Human.
Gene Name: apolipoprotein B
Protein Name: Apolipoprotein B-100

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450). Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: APOB (APOB Products)

Synonyms: FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody

Background: Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Synonyms: Apo B-100 | ApoB 100 | Apo B-48 | ApoB 48 | APOB | Apolipoprotein B 100 | Apolipoprotein B | Apolipoprotein B-48 | Apolipoprotein B48 | FLDB | LDLCQ4 | P04114

Gene ID: 338

UniProt: P04114

Pathways: Lipid Metabolism