CASR antibody (AA 926-1078)

Catalog No. ABIN4886502

This anti-CASR antibody is a Rabbit Polyclonal antibody detecting CASR in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for CASR antibody (AA 926-1078) (ABIN4886502)

Target: CASR (Calcium-Sensing Receptor (CASR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CASR Antibody

Binding Specificity: AA 926-1078

Purpose: Anti-Calcium Sensing Receptor/CASR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Calcium Sensing Receptor/CASR Antibody Picoband® (ABIN4886502). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Coburn JW, Elangovan L, Goodman WG, Fraza111 JM (Dec 1999). "Calcium-sensing receptor and calcimimetic agents". Kidney International. Supplement 73: S52-8. 2. D'Souza-Li L (Aug 2006). "The calcium-sensing receptor and related diseases". Arquivos Brasileiros De Endocrinologia E Metabologia 50 (4): 628-39. 3. Pidasheva S, Canaff L, Simonds WF, Marx SJ, Hendy GN (Jun 2005). "Impaired cotranslational processing of the calcium-sensing receptor due to signal peptide missense mutations in familial hypocalciuric hypercalcemia". Human Molecular Genetics 14 (12): 1679-90.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CASR antibody (ABIN4886502)

Yuan, Shi, Li, Yang, Tang, Hu, Du, Li, Gu, Wang, Xi, Tang: "Evaluation of a TPTX model induced by ischemia." in: Experimental animals, Vol. 66, Issue 4, pp. 345-356, (2018) (PubMed).

Target Details for CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Alternative Name: CASR

Background:

Synonyms: Extracellular calcium-sensing receptor,CaSR,Parathyroid cell calcium-sensing receptor 1,PCaR1,CASR,GPRC2A, PCAR1,

Tissue Specificity: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Background: The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.

Molecular Weight: 150 kDa

Gene ID: 846

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis