Catalase antibody (AA 344-527)

Catalog No. ABIN4886504

Product Details anti-Catalase Antibody

Target: Catalase (CAT)

Binding Specificity: AA 344-527

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Catalase(CAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Catalase(CAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: catalase
Protein Name: Catalase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT (CAT Products)

Synonyms: 2210418N07 antibody, Cas-1 antibody, Cas1 antibody, Cs-1 antibody, CS1 antibody, Cat01 antibody, Catl antibody, fb68a12 antibody, wu:fb68a12 antibody, CAT antibody, CATA antibody, CG6871 antibody, CT21282 antibody, CatA antibody, DMCATHPO antibody, DROCATHPO antibody, Dmel\\CG6871 antibody, U00145 antibody, bs36h11.y1 antibody, cat antibody, GB11648 antibody, catalase antibody, Cat antibody, BA0843 antibody, DKFZp469E0232 antibody, catalase antibody, Catalase antibody, catalase, gene 2 antibody, CAT antibody, Cat antibody, cat antibody, cat.2 antibody, LOC769804 antibody, BA_0843 antibody

Background: Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Synonyms: Cas1 | CAT | Catalase | Cs1 | P04040

Gene ID: 847

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism