GADD45A antibody (C-Term)

Catalog No. ABIN4886599

This Rabbit Polyclonal antibody specifically detects GADD45A in WB. It exhibits reactivity toward Human.

Quick Overview for GADD45A antibody (C-Term) (ABIN4886599)

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GADD45A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GADD45A Antibody

Binding Specificity: AA 125-165, C-Term

Purpose: Anti-GADD45A Antibody

Sequence: VLVTNPHSSQ WKDPALSQLI CFCRESRYMD QWVPVINLP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GADD45A Antibody (ABIN4886599). Tested in WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Alternative Name: GADD45A

Background:

Synonyms: Growth arrest and DNA damage-inducible protein GADD45 alpha,DNA damage-inducible transcript 1 protein,DDIT-1,GADD45A,DDIT1, GADD45,

Tissue Specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Background: Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. It is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. In addition, Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.

Molecular Weight: 19 kDa

Gene ID: 1647

UniProt: P24522

Pathways: p53 Signaling, Cell Division Cycle