Cytokeratin 5 antibody (Middle Region)

Catalog No. ABIN4886654

Product Details anti-Cytokeratin 5 Antibody

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Binding Specificity: AA 286-317, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Keratin, type II cytoskeletal 5(KRT5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KVELEAKVDA LMDEINFMKM FFDAELSQMQ TH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Keratin, type II cytoskeletal 5(KRT5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: keratin 5
Protein Name: Keratin, type II cytoskeletal 5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5 (286-317aa KVELEAKVDALMDEINFMKMFFDAELSQMQTH), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: KRT5 (KRT5 Products)

Synonyms: 3300001P10Rik antibody, AW146334 antibody, CK5 antibody, K5 antibody, Krt2-5 antibody, Tfip8 antibody, DDD antibody, DDD1 antibody, EBS2 antibody, KRT5A antibody, Sak57 antibody, ckii antibody, wu:fb02h09 antibody, wu:fj02d02 antibody, wu:fk78b12 antibody, zfCKII antibody, KRT75 antibody, CK-5 antibody, Keratin-5 antibody, keratin 5 antibody, Krt5 antibody, KRT5 antibody, krt5 antibody

Background: Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Synonyms: CK-5 | CK5 | Cytokeratin-5 | Cytokeratin5 | DDD | DDD1 | EBS2 | K5 | Keratin 5 | Keratin | Keratin-5 | Keratin5 | KRT 5 | Krt5 | KRT5A | P13647

Gene ID: 3852

UniProt: P13647