MAP3K8 antibody (AA 9-193)
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- Target See all MAP3K8 Antibodies
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
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Binding Specificity
- AA 9-193
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MAP3K8 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 8(MAP3K8) detection. Tested with WB, IHC-P in Human,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 8(MAP3K8) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: mitogen-activated protein kinase kinase kinase 8
Protein Name: Mitogen-activated protein kinase kinase kinase 8 - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193). Human MAP3K8 shares 89.7% and 90.3% amino acid (aa) sequence identity with mouse and rat MAP3K8, respectively.
- Isotype
- IgG
- Top Product
- Discover our top product MAP3K8 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
- Alternative Name
- MAP3K8 (MAP3K8 Products)
- Synonyms
- MAP3K8 antibody, COT antibody, EST antibody, ESTF antibody, MEKK8 antibody, TPL2 antibody, Tpl-2 antibody, c-COT antibody, Cot antibody, Cot/Tpl2 antibody, Est antibody, Estf antibody, Tpl2 antibody, D17TPL2 antibody, mitogen-activated protein kinase kinase kinase 8 antibody, MAP3K8 antibody, map3k8 antibody, Map3k8 antibody
- Background
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Mitogen-activated protein kinase kinase kinase 8 is an enzyme that in humans is encoded by the MAP3K8 gene. This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. Additionally, this protein was found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.
Synonyms: c COT | c-COT | CCOT | COT | EST | ESTF | MAP3K 8 | MAP3K8 | MEKK8 | TPL2 | TPL-2 | TPL 2 | P41279 - Gene ID
- 1326
- UniProt
- P41279
- Pathways
- PI3K-Akt Signaling, TCR Signaling
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