TUBB antibody (C-Term)

Catalog No. ABIN4886757

Cited in 3+ publications. The Rabbit Polyclonal anti-TUBB antibody (ABIN4886757) specifically detects TUBB in WB, IHC, FACS, IF and ICC. The antibody is reactive with Human, Mouse, Rat, Chicken and Monkey samples.

Quick Overview for TUBB antibody (C-Term) (ABIN4886757)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TUBB Antibody

Binding Specificity: C-Term

Purpose: Anti-Beta Tubulin/TUBB Antibody

Sequence: EQFTAMFRRK AFLHWYTGEG MDEMEFTEAE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-Beta Tubulin/TUBB Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-TUBB antibody (ABIN4886757)

Qu, Zhang, Du, Wang, Yang, Guo, Wang, Zhang, Xu: "Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 4254-4260, (2017) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Cao, Li, Li, Xiong, Zhou, Fan, Yu, Mao: "The potential role of HMGB1 release in peritoneal dialysis-related peritonitis." in: PLoS ONE, Vol. 8, Issue 1, pp. e54647, (2013) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB

Background:

Background: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Gene Full Name: tubulin beta class I

Molecular Weight: 50 kDa

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase